IUCN Otter Specialist Group . . . leading global otter conservation Last Update: Thursday November 22, 2018
 
 
[Home]

IUCN/SCC Otter Specialist Group Bulletin
©IUCN/SCC Otter Specialist Group

Volume 26 Issue 1 pages 1 - 62 (April 2009)

Citation: García, P., Arévalo, V. and Mateos, I. (2009). Using Sightings for Estimating Population Density of Eurasian Otter (Lutra lutra): a Preliminary Approach with Rowcliffe et al's Model . IUCN Otter Spec. Group Bull. 26 (1): 50 – 59

Previous | Contents | Next

Using Sightings for Estimating Population Density of Eurasian Otter (Lutra lutra): a Preliminary Approach with Rowcliffe et al's Model

Pablo García1, Valentín Arévalo2 and Isabel Mateos3

1Society for the Conservation of Vertebrates (SCV). Ave. Los Pinos, 17; 5°A. 28914 Leganés, Madrid, Spain. e-mail: garciap@usal.es
1Pza. de la Iglesia, 2. 47230 Matapozuelos, Valladolid, Spain
3 C/ Los Transportistas, 15; 4°A. 37006 Salamanca, Spain

Pablo Garcia (click for larger version)Valentin Arevalo (Click for larger version)Isabel Mateos (Click for larger version)

Abstract:Because of the crucial importance for otter ecology and conservation, estimating population size (or standardizing it in density) is one of the major fields of research. However, the behaviour of the species makes estimates complicated. The vast majority of methods rely on the study of signs (mainly spraints, but also tracks), but this is insufficient for density measurements, or too costly. Other techniques with proven results use visual counts of otters, but they also have some faults and involve a disproportionate sampling effort. The encounter-based method of population estimation, proposed by Rowcliffe et al. (2008), has been applied to otter sightings in two sites of different habitat with known otter density, and under different survey designs. This provisional check appears to confirm that the method results in adequate population estimates, also when employing samples at random intervals of time and space. Moreover, it involves lower effort, and the census can be tackled by a reduced number of researchers. It is strongly suggested that this methodology be widely tested, to ascertain its performance under different sampling and environmental conditions.

Keywords: central Spain, density, encounter-based model, spatial ecology, survey method.
Française | Español

INTRODUCTION

Estimating density of wild populations of the Eurasian otter (Lutra lutra) is very difficult, because of its cryptic and mainly nocturnal habits (Mason and Macdonald, 1986, 1987; Kruuk, 1995, 2006; Wilson et al., 1996; Wilson and Delahay, 2001; Chanin, 2003; Long et al., 2008). In the past, therefore, abundance data have been obtained from spraint (otter faeces) numbers or, more roughly, from the number of positive sites, i.e. with spraints and/or tracks, registered during surveys (Mason and Macdonald, 1986, 1987, 2004; Kruuk, 1995, 2006; Reuther et al., 2002; Chanin, 2003; Gruber et al., 2008; Roberts et al., 2008). However, there is an considerable amount of evidences that criticises the use of faeces for this purpose (Kruuk et al., 1986; Kruuk and Conroy, 1987; Kruuk, 1992, 1995, 2006; Reuther et al., 2002; Gallant et al., 2007; Roberts et al., 2008). Criticisms are based on data indicating that the number of spraints produced by an otter varies on a seasonal basis, and sign quantities in a particular place are nor indicative of neither animal abundance nor of activity there (see references above).

Recently, Guter et al. (2008) have tried to demonstrate that spraint counts correlated with activity of an otter in a given site, but their results are questionable for several reasons. Gruber et al. (2008) have built a maximum likelihood model to estimate daily activity rates from the presence of aged and/or fresh signs, although this has not been tested yet.

Several methods have been developed and tested to accurately measure otter density, using counts from infra-red machines or with DNA-based identification of hairs and spraints (Hung et al., 2004; Waits and Paetkau, 2005; Garcia et al., 2006; Kalz et al., 2006; Prigioni et al., 2006a; Arrendal et al., 2007; Lanszki et al., 2008; Hájková et al., 2008). These methods, although being useful, are expensive and they are likely to have some disadvantages (Chanin, 2003; Waits and Paetkau, 2005; Kruuk, 2006).

In coastal environments, Kruuk et al. (1989), and Yoxon (2003), aproximate the number of otters occurring in an area by counting den sites (“holts”), which relate to population size. This is not useful in freshwater ecosystems, as the number of den sites can be misidentified if one does not use radio-tracking (Kruuk et al., 1998).

Northern areas of the north hemisphere have snow cover during long times of the year, and snow-tracking is a “standard” method for successfully estimating otter populations (Sidorovich and Macdonald, 2001; Chanin, 2003; Arrendal et al., 2007; Sulkava, 2007; Sulkava and Liukko, 2007; Hájková et al., 2008). 

It is obvious that the most accurate way for studying otter populations is by using direct observations (Kruuk and Moorhouse, 1991; Kruuk, 1995, 2006; Ruiz-Olmo, 1995; Wilson et al., 1996; Bravo et al., 1998; Ruiz-Olmo et al., 2001; Wilson and Delahay, 2001; Saavedra, 2002; Chanin, 2003). This method requires otters to be active in daylight, and needs individual identification of animals. The occurrence of a certain degree of diurnal activity of the otter in the Mediterranean basin enables censuses from direct, visual observations (Ruiz-Olmo, 1995; Bravo et al., 1998; Ruiz-Olmo et al., 2001; Saavedra, 2002). However, the fact that otters do not have individually distinctive markings in this area (Kruuk, 1995, 2006; but see a possibility in Gilkinson et al., 2007) makes this kind of study more complex, and for the procedure (Ruiz-Olmo, 1995), it is necessary to mobilize a large quantity of resources, and the use of inexperienced volunteers (Bravo et al., 1998), to simultaneously cover the study area.

This methodology has produced good estimates of the number of otters in a locality (Ruiz-Olmo, 1995; Bravo et al., 1998; Ruiz-Olmo et al., 2001; Saavedra, 2002), but visibility of the animals may be a problem (Walsh et al., 2009), and a good view of the sampling area is necessary. Often, riparian woodland affects the detection of individuals, but this can be corrected by increasing the number of people taking part in the work.

The aim of the present study is to test the effectiveness of this method based on direct observations, but without involving considerable logistic effort.

MATERIAL AND METHODS

Study areas

To test the adjustment of the design (see Figure 1 below), two sites with quite different environmental attributes were selected in the province of Salamanca (central Spain). In those two areas the climate is continental Mediterranean, with cold and wet winters but warm and dry summers. Both sites show degraded environmental characteristics, as a consequence of uncontrolled human activity.

The first area is the Riolobos reservoir, an enclave in the steppe of the province of Salamanca (41º 0’ 24.38’’ N 5º 18’ 12.93’’ W, 833 m.a.s.l., extent: about 0.20 km2Rouco, 2006). This dam was built in the 90’s and has been used since 2000, being colonized by otters in recent times, in 2005 (Rouco, 2006). The shape of this reservoir is approximately circular, with some peninsulas making the profile somewhat complex. The muddy and sandy shores of Riolobos lack any vegetation cover, with an arid band from the water over three to six meters. Riolobos reservoir is the only area holding permanent water during winter in this area, thus surveys were focused here.

Riolobos has been one of the most important areas for aquatic birds in Central Spain and the most important in Salamanca (Rouco, 2006), but because of the management of fisheries in the reservoir by the regional administration, nowadays the Riolobos’ biodiversity has been largely lost (Rouco, 2006). For Eurasian otters, human pressure on the water complexes of the area, nearly all from fishermen, is the key factor limiting the establishment of a population (own data).

The second locality is the river Tormes where it runs through the city of Salamanca (40º 57’ 23.25’’ N 5º 39’ 40.59’’ W, 800 m.a.s.l., about 1.49 km2). This is a linear habitat, with banks covered with a thick riparian forest, sometimes rather thin as a consequence of the recreational activities of people in the riverine area. The river is wide, maximally about 400 m, with many islands, and low gradient (< 2 %). Several small tributaries are also present. The river is highly polluted, although this does not appear to affect the occurrence of otters.

The presence of otters in these areas is seasonal, restricted to winter and/or fall (Rouco, 2006; García et al., 2009; own data). For more details on these sites see Rouco (2006), García (2008), García and Mateos (2009), and García et al. (2009).

Otter surveys

The basic technique consists in the observation of a water surface from an elevated point, with binoculars and telescope, for a given time looking for otter activity. These observations were carried out at dusk and dawn, when it is more likely that otters are active. The basic unit of sampling effort is one hour per observer.

In Riolobos, because of its shape and small area, the entire water surface can be monitored from only two points, one on the wall of the dam, and another one an elevated point along the shore (Ruiz-Olmo, 1995; Ruiz-Olmo et al., 2001). From January to February 2009, ten periods of observations were carried out at this site, when the entire surface was monitored simultaneously by the observers. Due to the absence of neighbouring water bodies, the movements of otters seem to be restricted to this site.

The river Tormes in Salamanca is different. The lineal structure of the habitat, and the relative high forest cover in some sites, reduces the probabilities of encounters, and the area must be monitored by a larger number of observers. Here, the model (briefly described down here; Rowcliffe et al., 2008) was checked for another objective: to confirm if it is possible to estimate the density of otters from a relatively high number of independent sampling sites, not covering the entire area all at the same time.

Fourteen sites were defined for this purpose (mostly on bridges), from which the observations were made, and monitoring was repeated at least three times. Stretches were surveyed from January to February 2009, on days selected randomly. Evidences show that otters also use the available streams in the area, so this habitats were also sampled.

Length of the sites surveyed was setted in a minimum of 10 km following the recommendation of Ruiz-Olmo (1995), allowing a representative sample of the population abundance.

The number of observations during each period were divided by the units of sampling effort (one hour per observer) for standardizing the results.

Independently, the population of otters was estimated, for the dates when the studies were carried out, from two widely used methods and in the same surface sampled through sightings. In Riolobos, as the environmental conditions allow this, animals were censused from direct observations at dawn and daybreak. Additionally, other population size estimate came from mud fresh tracks found in the ten sessions, after carrying out the vigils (esimation by measuring some track parameters and analysed by one-way ANOVA and discriminant analysis; see further details in: Ruiz-Olmo et al., 2001; Saavedra, 2002; Mercier and Fried, 2003; Gusset and Burgener, 2006; García, 2008). In Salamanca, otter population was estimated also from this kind of mud and snow tracking following the same guidelines for analysis.

Sampling the same surfaces at the same time with different population size estimation techniques allow for testing the fitness of the methods.

RFTC model and estimation of parameters

Rowcliffe et al. (2008) developed a model (hereafter, RFTC model), that estimates carnivore density from encounter rates of animals by camera traps, and this is potentially suitable for application to otter sightings. An observer with adequate equipment works in a similar way to camera traps, covering a surface of the water body with the shape of a part of a circle, the detection area (Figure 1). Thus, this detection area could be defined by its radius (r) and the angle of observation (Θ) (Figure 1; Rowcliffe et al., 2008). Density with this formula is defined in relationship with the observation rate (number of observations, y, per unit of time effort, t; see down here):

D (animals/km2) = y/t π/vr(2 + Θ)

being v the velocity of the movements (km/h) of animals during the sampling period. This speed was measured from the observations obtained in the field work, 1.96 km/h in Salamanca and 1.67 km/h in Riolobos. Other available calculations of this parameter (Kruuk, 1995, 2006; Ruiz-Olmo, 1995; Saavedra, 2002; Garcia et al., 2006; Prigioni et al., 2006b) were not employed, given that it varies within the range of the otter, and it is better to use data from the populations studied. This value is easy to obtain from the visual censuses or deriving from parsimonious approximations (Tremblay et al., 2009).

Diagram of observation angle incidence onto the observation point
Figure 1. An example of the design and measurement of parameters employed in the RFTC formula: (r): radius; (Θ): angle of observation.

The radius (r) and the angle (Θ) of the detection area were estimated on aerial photographs from the sites. The radius (expressed in kilometers) was defined as the maximum length that can be viewed from a point. As there are two, left and right, radia, both were measured and the mean value used. The effective angle (radians) of detection was calculated from aerial photographs using ImageJ software (http://rsbweb.nih.gov/ij/).

If otters were watched during a large time, an independent observation (as defined previously for the model) was considered each 20 minutes (Rowcliffe et al., 2008).

The density of otters for Riolobos was independently measured for the ten surveys, and calculated by summing the density obtained by the observers. For Salamanca the design and the analysis were different. As samples were taken at random intervals, both in time and space, the density (otters/km2) was also estimated independently for each of the 14 sites sampled. After that, a mean otter density from this data was calculated (see procedures in the next section), and the population size simply estimated by multiplying this mean density for the total surface studied.

Statistical analysis
Bootstrap is a resampling method used for assessing the descriptive parameters of a sample with an unknown statistical distribution, working well with animal density estimations (Stephens et al., 2006). A bootstrap procedure was employed for estimating the mean and deviation parameters of the density estimators, considering in Riolobos two subsamples of five sets of observations, and two subsamples with seven localities in Salamanca. In both cases, 5000 replicates were employed for the bootstrap. In Riolobos, this was used for estimating the otter population density from visual counts and with the RFTC model, whilst for Salamanca it was used for estimating the mean surveyed site RFTC otter density.

The estimates from direct counts, mud tracking and from the RFTC equation in Riolobos were correlated using the non-parametric Spearman rank (RS). All statistical calculations were carried out with S-PLUS 8.0 and MyStat 12.0.

RESULTS

The numbers of Eurasian otters inhabiting Salamanca and Riolobos, as estimated, respectively, from tracking and visual census, were two animals for both sites, an abundance of 0.14 and 0.20 otters/km of bank.

For the area of Riolobos, the otter density derivated from the RFTC equation compares well with the visual count and mud tracking data, and the correlation is statistically highly significant (Visual counts vs. RFTC model: RS = 0.99, d.f. = 9, P=0.0001; Mud tracking vs. RFTC model: RS = 0.98, d.f. = 9, P=0.0001). The bootstrap density estimates assessed from these methods are similar [expressed in otters/km2; direct census (mean ± SE, 95% CI): 0.40 ± 0.57, lower: 0.00, upper: 0.80; RFTC density (mean ± SE, 95% CI): 0.40 ± 0.56, lower: 0.00, upper: 0.79).

For the locality of Salamanca, the mean RFTC bootstrapped estimate for the 14 river stretches sampled was 1.41 ± 0.12 otters/km2 (mean ± SE; 95% CI: lower: 1.33, upper: 1.50 otters/km2), similar to, or perhaps a little higher, than the value from tracking, nearly 1.34 otters/km2. The overall population size in Salamanca was 1.92 from the RTFC model and two otters from tracks.

Table 1: Parameters measured for the RFTC equations in the two areas studied.
Locality

Otter velocity (km/h)

Mean r (km) Mean Θ (radians)

Riolobos 1.67 0.55 0.89
Salamanca 1.96 0.34 0.61

DISCUSSION

The results presented here appear to confirm that the RFTC model is a useful way for estimating populations of otters, as well as making the census easier.

The method has a lot of potential advantages compared to those widely used for studying otters: i) it provides a reliable approximation of the otter populations in different environments; ii) it corrects the potential biases due to a lack of visual sightings (Walsh et al., 2009) by considering only the surface of the area watched, and not assuming that the probability of detection is always equal; iii) it is a cost-effective technique that does not require the help of a high number of personnel (sometimes inexperienced); and iv) the most interesting and outstanding aspect of the RFTC, as appears in the analysis, is that it is possible to take independent samples, in time and space, of the area of interest by the same researcher. Last but not least, the density estimate compares well with the actual status.

These properties are not so clearly defined in the alternative methods for censusing otter populations. Spraint-based analysis, probably including molecular scatology, has numerous faults and/or it costs too much, nearly €60 / $80.40 and around 12 hours/person of work for each spraint, including those that do not give positive results (Mason and Macdonald, 1986, 1987; Kruuk et al., 1986; Kruuk and Conroy, 1987; Kruuk, 1992, 1995, 2006; Reuther et al., 2002; Chanin, 2003; Hung et al., 2004; Waits and Paetkau, 2005; Garcia et al., 2006; Kalz et al., 2006; Prigioni et al., 2006a; Arrendal et al., 2007; Gallant et al., 2007; Gruber et al., 2008; Hájková et al., 2008; Lanzski et al., 2008; Roberts et al., 2008).

Snow and sand/mud tracking can fail in particular situations (probably corrected by increasing and improving the quality of the sample and the robustness of statistical analysis; Ruiz-Olmo et al., 2001; Sidorovich and Macdonald, 2001; Chanin, 2003; Stephens et al., 2006; Arrendal et al., 2007; Sulkava, 2007; Sulkava and Liukko, 2007; García, 2008; Gruber et al., 2008; Hájkova et al., 2008). Visual counts (Ruiz-Olmo, 1995), in spite of enabling researchers to get quality ecological and abundance data (Bravo et al., 1998; Ruiz-Olmo et al., 2001; Saavedra, 2002), need a large number of observers (at least 20 people for 10 km of river) during several hours, without any guarantee of success. These drawbacks seem to be, at least partially, removed when applying the RFTC formula.

The major constraint of the RFTC method, shared with direct census (Ruiz-Olmo, 1995; Ruiz-Olmo et al., 2001; Saavedra, 2002), is its dependence on diurnal activity of otters. Otters are mainly nocturnal, but in the Mediterranean basin the activity pattern contains a certain degree of daylight movements (Ruiz-Olmo, 1995; Ruiz-Olmo et al., 2001; Saavedra, 2002), making counts possible. Perhaps this does not happen in all populations (but see Kruuk and Moorhouse, 1991; Kruuk, 1995, 2006), and it is advisable to carry out a pilot study in advance of the main fieldwork, to evaluate the presence of dusk and dawn activity. Increasing the number of visits to a sampling site will improve the precision of the results.

As demonstrated by our results, the RFTC equation appears to constitute a valuable option for estimating otter populations. However, it should be pointed out that this is only an initial check of this model for this specific goal, and our sample size is small. Indeed, more calibrations are needed in otter populations of known size (Wilson et al., 1996; Wilson and Delahay, 2001). Some provisional data in other localities in central Spain tend to confirm the present observations (own data).

We strongly encourage to further tests of the RFTC effectiveness, improving its efficiency and correcting faults, as well as exposing its robustness (Wilson et al., 1996; Wilson and Delahay, 2001; Long et al., 2008). Specially, one needs to acquire insights into its precision and biasses when using independent samples at given time intervals (Stanley and Royle, 2005). If necessary, further factors (e.g., correction coefficients for the diurnal activity, or for the time intervals of sampling; Stanley and Royle, 2005) and changes may be usefully introduced in the formula.

It is also possible that the method becomes an option for other otter species with daylight activity (Kruuk, 2006), and in marine populations of the Eurasian otter (Kruuk and Moorhouse, 1991; Yoxon, 2003).

AKNOWLEDGEMENTS - Thanks to L. Pérez, D. Díaz, P. García and C. Ayres for their help and support in various ways. We also want to greatly acknowledge the comments of the reviewer and his help with the English speaking. On 9th April 2009, an otter cub was found dead as a road casualty near to Riolobos reservoir; in the first year that otters bred in the area. It is very probable, as suggested in our results (see also Rouco, 2006, for birds), that the disturbance by fishing people in an area without enough den sites, requires otters to disperse to an adequate site for rearing cubs, because in the area fish are abundant, and therefore not likely to limit numbers of breeding otters, as happens in the Mediterranean basin. As the problems caused by fishing people are a consequence of an unfortunate, debatable decision by the local environmental administration, we want to dedicate this work to the animals inhabiting Riolobos, which are surviving against the odds.

REFERENCES

Arrendal, J., Vilà, C., Björklund, M. (2007). Reliability of noninvasive genetic census of otters compared to field censuses. Conserv. Genet. 8: 1097-1107.
Bravo, C., Bueno, F., Sánchez-Aguado, F. (1998). I Censo visual de nutria (Lutra lutra) y visón americano (Mustela vison) en el Parque Natural de las Hoces del Duratón (Segovia). Galemys 10: 89-96.
Chanin, P. (2003). Monitoring the otter Lutra lutra. Conserving Natura 2000 Rivers Monitoring Series 10: 1-43.
Gallant, D., Vasseur, L., Bérubé, C.H. (2007). Unveiling the limitations of scat surveys to monitor social species: a case study on river otters. J. Wildl. Manag. 71: 258-265.
Garcia, C., Forman, D.W., Davies, S., Thomson, A. (2006). Non-intrusive monitoring of otters (Lutra lutra) using infrared technology. J. Zool. 270: 677-684.
García P. (2008). Abundance of the Eurasian Otter Lutra Lutra (Linnaeus, 1758) in two areas from Central Spain (province of Salamanca, river Tormes). IUCN Otter Spec. Group Bull. 25: 32-37.
García, P., Mateos, I. (2009). Evaluation of three indirect methods for surveying the Least weasel (Mustela nivalis) in a Mediterranean area. Small Carniv. Conserv. 40: 22-26.
García, P., Ayres, C., Mateos, I. (2009). Seasonal changes in American mink (Neovison vison) signs related to Eurasian otter (Lutra lutra) presence. Mammalia 73: in press.
Gilkinson, A.K., Pearson, H.C., Weltz, F., Davis, R.W. (2007). Photo-identification of sea otters using nose scars. J. Wildl. Manag. 71(6): 2045-2051.
Gruber, B., Reineking, Calabrese, J.M., Kranz, A., Poledníkova, K., Poledník, L., Klenke, R., Valentin, A., Henle, K. (2008). A new method for estimating visitation rates of cryptic animals via repeated surveys of indirect signs. J. Appl. Ecol. 45: 728-735.
Gusset, M., Burgener, N. (2006). Estimating larger carnivore numbers from track counts and measurements. Afr. J. Ecol. 43: 320-324.
Guter, A., Dolev, A., Saltz, A., Kronfeld-Schor, A. (2008). Using videotaping to validate the use of spraints as an index of Eurasian otter (Lutra lutra) activity. Ecol. Indic. 8: 462-465.
Hájková, P., Zemanová, B., Roche, K., Hájek, B. (2008). An evaluation of field and noninvasive genetic methods for estimating Eurasian otter population size. Conserv. Genet. DOI 10.1007/s10592-008-9745-4.
Hung, C.M., Li, S.-H., Lee, L.-L. (2004). Faecal DNA typing to determinate the abundance and spatial organisation of otter (Lutra lutra) along two stream systems in Kinmen. Anim. Conserv. 7: 301-311.
Kalz, B., Jewgenow, K., Fickel, J. (2006). Structure of an otter (Lutra lutra) population in Germany – results of DNA and hormone analyses from faecal samples. Mamm. Biol. 71: 321-335.
Kruuk, H. (1992). Scent marking by otters (Lutra lutra): signaling the use of resources.Behav. Ecol. 3: 133-140.
Kruuk, H. (1995). Wild otters: predation and populations. Oxford University Press, Oxford, UK.
Kruuk, H. (2006). Otters. Ecology, behaviour and conservation. Oxford University Press, Oxford, UK.
Kruuk, H., Conroy, J.W.H., Glimmerveen, U., Ouwerkerk, E.J. (1986). The use of spraints to survey populations of otters Lutra lutra. Biol. Cons. 35: 187-194.
Kruuk, H., Conroy, J.W.H. (1987). Surveying otter Lutra lutra populations : a discussion of problems with spraints. Biol. Conserv. 41: 179-183.
Kruuk, H., Moorhouse, A. (1991). The spatial organization of otters (Lutra lutra L.) in Shetland. J.  Zool. 224: 41-57.
Kruuk, H., Moorhouse, A. & Conroy, J.W.H., Durbin, L., Frears, S. (1989). An estimate of numbers and habitat preference of otters Lutra lutra in Shetland, UK. Biol. Cons. 49: 241-254.
Kruuk, H., Carss, D.N., Conroy, J.W.H., Gaywood, M.J. (1998). Habitat use and conservation of otters (Lutra lutra) in Britain: a review. In: Dunstone N., Gorman M. (Eds.), Behaviour and ecology of riparian mammals. Cambridge University Press, Great Bretain, pp. 119-134.
Lanszki, J., Hidas, A., Szentes, K., Révay, T., Lehoczky, I., Weiss, S. (2008). Relative spraint density and genetic structure of otter (Lutra lutra) along the Drava river in Hungary. Mamm. Biol. 73: 40-47.
Long, R., MacKay, P., Ray, J., Zielinski, W. 2008. Noninvasive survey methods for carnivores. Island Press, 400 pp.
Mason, C.F., Macdonald, S.M. (1986). Otters, conservation and ecology. Cambridge University Press, Cambridge, UK.
Mason, C.F., Macdonald, S.M. (1987). The use of spraints for surveying the otter Lutra lutra populations: an evaluation. Biol. Cons. 41: 167-177.
Mason, C.F., Macdonald, S.M. (2004). Growth in otter (Lutra lutra) populations in the UK as shown by long-term monitoring. Ambio 33: 148-152.
Mercier, L., Fried, G. (2003). Preliminary study of otter (Lutra lutra) prints in captivity as a tool for field research. In: Conroy, J.W.H., Gutleb, A.C., Ruiz-Olmo, J., Yoxon, G.M. (Eds). Proceedings of the European Otter conference “Return of the otter in Europe – where and how?”. Journal of the International Otter Survival Fund no. 2. Isle of Skye, 30th June – 5 Jule 2003. IOSF.
Prigioni, C., Remonti, L., Balestrieri, Sgrosso, S., Priore, G., Mucci, N., Randi, E. (2006a). Estimation of European otter (Lutra lutra) population size by fecal DNA typing in southern Italy. J. Mammal. 87: 855-858.
Prigioni, C., Remonti, L., Balestrieri, A. (2006b). Otter Lutra lutra movements assessed by genotyped spraints in souther Italy. Hystrix, It. J. Mammal. 17: 91-96.
Reuther, C., Dolch, D., Green, R., Jahrl, J., Jefferies, D., Krekemeyer, A., Kucerova, M., Madsen, A.B., Romanowski, J., Röche, K., Ruiz-Olmo, J., Teubner, J., Trindade, A. (2002).  Surveying and monitoring distribution and population trends of the Eurasian Otter (Lutra lutra). Guidelines and evaluation of the standard method surveys as recommended by the European Section of the IUCN/SSC Otter Specialist Group. Habitat 12: 1-148.
Roberts, N.M., Crimmins, S.M., Hamilton, D.A., Gallagher, E. (2008). An evaluation of bridge-sign surveys to monitor river otter (Lontra canadiensis) populations. Am. Midl. Nat. 160: 358-363.
Rouco, M. (2006). Las aves del azud de Riolobos (y de su entorno). SEO/BirdLife-Grupo Local SEO-Salamanca. Fundación Germán Sánchez-Ruipérez, Peñaranda de Bracamonte, Spain.
Rowcliffe, J.M., Field, J., Turvey, S.T., Carbone, C. (2008). Estimating animal density using camera traps without the need for individual recognition. J. App. Ecol. 45: 1228-1236.
Ruiz-Olmo, J. (1995). Visual census of Eurasian otter (Lutra lutra): a new method. Habitat 11: 125-130.
Ruiz-Olmo, J., Saavedra, D., Jiménez, J. (2001). Testing the surveys and visual and track censuses of Eurasian otters (Lutra lutra). J. Zool. 253: 359-369.
Saavedra, D. (2002). Reintroduction of the Eurasian Otter (Lutra lutra) in Muga and Fluvià basins (North-Eastern Spain): viability, development, monitoring and trends of the new population. Tesis doctoral. Universidad de Girona, Girona, Spain.
Sidorovich, V., Macdonald, D.W. (2001). Density dynamics and changes in habitat use by the European mink and other native mustelids in connection with the American mink expansion in Belarus. Neth. J. Zool. 51: 107-126.
Stanley, T.R., Royle, J.A. (2005). Estimating site occupancy and abundance using indirect detection indices. J. Wildl. Manag. 69: 874-883.
Stephens, P.A., Zaumyslova, O.Y., Miquelle, D.G., Myslenkov, A.I., Hayward, G.D. (2006). Estimating population density from indirect sign: track counts and the Formozov-Malyshev-Pereleshin formula. Anim. Conserv. 9: 339-348.
Sulkava, R. (2007). Snow tracking: a relevant method for estimating otter Lutra lutra populations. Wildlife Biol. 13: 208-218.
Sulkava, R., U.M. Liukko, U.M. (2007). Use of snow-tracking methods to estimate the abundance of otter (Lutra lutra) in Finland with evaluation of one-visit census for monitoring purposes. Ann. Zool. Fenn,  44: 179-188.
Tremblay, Y., Robinson, P.W., Costa, D.P. (2009). A parsimonious approach to modeling animal movement data. PLoS ONE 4: 1-11.
Waits, L.P., Paetkau, D. (2005). Noninvasive genetic sampling tools for wildlife biologists: a review of applications and recommendations for accurate data collection. J. Wildl. Manag. 69: 1419-1433.
Walsh, D.P., Page, C.F., Campa, III, H., Winterstein, S.R. (2009). Incorporating estimates of group size in sightability models for wildlife. J. Wildl. Manag. 73(1): 136-143.
Wilson, G.J., Delahay, R.J. (2001). A review of methods to estimate the abundance of terrestrial carnivores using field signs and observations. Wildl. Res. 28(2): 151-164.
Wilson, D.E., Rusell, F., Nichols, J.D., Rudran, R., Foster, M.S. (1996). Measuring and monitoring biological diversity. Standard methods for mammals. Smithsonian Institution Press, Washington D.C.
Yoxon, P. (2003). Otter surveys of north west Scotish islands. In: Conroy, J.W.H., Gutleb, A.C., Ruiz-Olmo, J. and Yoxon, .G.M. (Eds). Proceedings of the European Otter conference “Return of the otter in Europe – where and how?”. Journal of the International Otter Survival Fund no. 2. Isle of Skye, 30th June – 5 Jule 2003. IOSF.

Résumé : Utilisation des Observations Visuelles pour Estimer la Densité de Population de la Loutre d'Europe (Lutra lutra): Étude Préliminaire à l'Aide du Modèle de Rowcliffe et al.
En raison de son importante capitale pour l’écologie et la conservation de la Loutre, l’estimation de la taille d’une population (ou la standardiser sous forme de densité) est l’un des meilleurs champs d’investigation actuel. Cependant, le comportement de cette espèce fait que cela devient très compliqué. La plupart des méthodes utilisent l'étude des traces (principalement les épreintes, mais aussi les empreintes) qui ne sont pas toujours suffisamment fiables ou alors trop coûteuses pour les mesures de densités. D’autres techniques montrent de bons résultats en utilisant le recensement visuel mais elles présentent des biais et requièrent un effort d'échantillonnage disproportionné. La méthode d’estimation de dénombrement d’une population proposée par Rowcliffe et al. (2008) a été appliquée aux observations directes de loutres sur deux sites d’habitats bien différents et de densités connues et ce avec plusieurs modèles. Les résultats préliminaires semblent confirmer l’efficacité de la méthode en obtenant une estimation similaire à la population connue, même en usant d’échantillons à des intervalles de temps et d'espace totalement aléatoires. Par ailleurs, le déploiement logistique est inférieur et le recensement peut être réalisé par un nombre réduit d'enquêteurs. Il est enfin recommandé de tester cette méthodologie de façon plus étendue afin d’en prouver les performances dans des conditions environnementales et des échantillons bien différents.
Revenez au dessus

Resumen: Empleo de Observaciones para Estimar la Densidad Poblacional de la Nutria Paleártica (Lutra Lutra): una Evaluación Preliminar Utilizando el Modelo de Rowcliffe et al.
Debido a su crucial importancia para la ecología y conservación de la nutria, estimar el tamaño poblacional (o estandarizarlo en densidad) es uno de los mayores campos de investigación. Sin embargo, el comportamiento de la especies hace que esto sea muy complicado. La mayor parte de los métodos dependen del estudio de los rastros (principalmente excrementos, pero también huellas), que no son siempre lo suficientemente robustos para mediciones de densidad, o bien son muy costosos. Otra técnica con buenos resultados demostrados utiliza censos visual de nutrias, pero tiene algunos fallos y requiere un esfuerzo de muestreo desproporcionado. El método basado en encuentro propuesto por Rowcliffe et al. (2008) ha sido aplicado a las observaciones directas de nutrias en dos sitios con diferente naturaleza y densidad de nutrias conocida, así como con diferentes diseños de muestreo. Esta revisión preliminar parece confirmar que este diseño permite obtener estimas de población adecuadas, incluso empleando muestras en intervalos al azar, tanto en el tiempo como en el espacio; además, el despliegue logístico es inferior, y el censo puede realizarse por un número reducido de investigadores. Se recomienda evaluar esta metodología ampliamente para establecer el rendimiento del método en condiciones ambientales y de muestro variables.
Vuelva a la tapa

Previous | Contents | Next